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T. Ge, D.G. Brickner, K. Zehr, D.J. VanBelzen, W. Zhang, C. Caffalette, S. Ungerleider, N. Marcou, B. Chait, M.P. Rout, J.H. Brickner. “Exportin-1 functions as an adaptor for transcription factor-mediated docking of chromatin at the nuclear pore complex”, bioRxiv. 2024.05.09.593355. [PMID: 38798450]

N.E. Ketaren, F.D. Mast, P.C. Fridy, J.P. Olivier, T. Sanyal, A. Sali, B.T. Chait, M.P. Rout, J.D. Aitchison. "Nanobody repertoire generated against the spike protein of ancestral SARS-CoV-2 remains efficacious against the rapidly evolving virus", Elife. 2024 May 7;12:RP89423. [PMID: 38712823]

E.R. Butterfield, S.O. Obado, S.R. Scutts, W. Zhang, B.T. Chait, M.P. Rout, M.C. Field. “A lineage-specific protein network at the trypanosome nuclear envelope”, Nucleus. 2024 Dec;15(1):2310452. Epub 2024 Apr 11. [PMID: 38605598]

D. Singh, N. Soni, J. Hutchings, I. Echeverria, F. Shaikh, M. Duquette, S. Suslov, Z. Li, T. van Eeuwen, K. Molloy, Y. Shi, J. Wang, Q. Guo, B. T. Chait, J. Fernandez-Martinez, M. P. Rout, A. Sali, E. Villa. "The Molecular Architecture of the Nuclear Basket", bioRxiv. 2024.03.27.587068. [PMID: 38586009]

J.R. Ramsey, P.M.M. Shelton, T.K. Heiss, P.D.B. Olinares, L.E. Vostal, H. Soileau, M. Grasso, S.W. Casebeer, S. Adaniya, M. Miller, S. Sun, D.J. Huggins, R.W. Myers, B.T. Chait, E.V. Vinogradova, T.M. Kapoor. “Using a function-first 'scout fragment'-based approach to develop allosteric covalent inhibitors of conformationally dynamic helicase mechanoenzymes”, J Am Chem Soc. 2024 Jan 10;146(1):62-67. [PMID: 38134034]


G.I. Small, O. Fedorova, P.D.B. Olinares, J. Chandanani, A. Banerjee, Y.J. Choi, H. Molina, B.T. Chait, S.A. Darst, E.A. Campbell. “Structural and functional insights into the enzymatic plasticity of the SARS-CoV-2 NiRAN domain”, Mol Cell. 2023 Oct 24. [PMID: 37890482]

C.W. Akey, I. Echeverria, C. Ouch, I. Nudelman, Y. Shi, J. Wang, B.T. Chait, A. Sali, J. Fernandez-Martinez, M.P. Rout. “Implications of a multiscale structure of the yeast nuclear pore complex”, Mol Cell. 2023 Sep 21; 83(18):3283-3302. [PMID: 37738963]

Taylor MS, Wu C, Fridy PC, Zhang SJ, Senussi Y, Wolters JC, Cajuso T, Cheng WC, Heaps JD, Miller BD, Mori K, Cohen L, Jiang H, Molloy KR, Chait BT, Goggins MG, Bhan I, Franses JW, Yang X, Taplin ME, Wang X, Christiani DC, Johnson BE, Meyerson M, Uppaluri R, Egloff AM, Denault EN, Spring LM, Wang TL, Shih IM, Fairman JE, Jung E, Arora KS, Yilmaz OH, Cohen S, Sharova T, Chi G, Norden BL, Song Y, Nieman LT, Pappas L, Parikh AR, Strickland MR, Corcoran RB, Mustelin T, Eng G, Yilmaz OH, Matulonis UA, Skates SJ, Rueda BR, Drapkin R, Klempner SJ, Deshpande V, Ting DT, Rout MP, LaCava J, Walt DR, Burns KH. “Ultrasensitive detection of circulating LINE-1 ORF1p as a specific multi-cancer biomarker”, Cancer Discovery. 2023 Sep 12. Online ahead of print. [PMID: 37698949]

G.N.L. Chua, J.W. Watters, P.D.B. Olinares, J.A. Luo, B.T. Chait, S. Liu. “Differential dynamics specify MeCP2 function at methylated DNA and nucleosomes”, bioRxiv. 2023 Jun 5: 2023.06.02.543478. Preprint. [PMID: 37333354]

T. Kozai, J. Fernandez-Martinez, T. van Eeuwen, P. Gallardo, L.E. Kapinos, A. Mazur, W. Zhang, J. Tempkin, R. Panatala, M. Delgado-Izquierdo, B. Raveh, A. Sali, B.T. Chait, L.M. Veenhoff, M.P. Rout, R.Y.H. Lim. “Dynamic molecular mechanism of the nuclear pore complex permeability barrier”, bioRxiv. 2023 Apr 14: 2023.03.31.535055. Preprint. [PMID: 37066338]

A. Gkeka, F. Aresta-Branco, G. Triller, E.P. Vlachou, M. van Straaten, M. Lilic, P.D.B. Olinares, K. Perez, B.T. Chait, R. Blatnik, T. Ruppert, J.P. Verdi, C.E. Stebbins, F.N. Papavasiliou. “Immunodominant surface epitopes power immune evasion in the African trypanosome”, Cell Rep. 2023 Mar 20. Online ahead of print. [PMID: 36943866]

Y. Udi, W. Zhang, M.E. Stein, I. Ricardo-Lax, H.A. Pasolli, B.T. Chait, M.P. Rout. “A general method for quantitative fractionation of mammalian cells”, J Cell Biol. Epub 2023 Mar 15. [PMID: 36920247]

B.F. Malone, J.K. Perry, P.D.B. Olinares, H.W. Lee, J. Chen, T.C. Appleby, J.Y. Feng, J.P. Bilello, H. Ng, J. Sotiris, M. Ebrahim, E.Y.D. Chua, J.H. Mendez, E.T. Eng, R. Landick, M. Götte, B.T. Chait, E.A. Campbell, S.A. Darst. “Structural basis for substrate selection by the SARS-CoV-2 replicase”, Nature. 2023 Feb 1. Online ahead of print. [PMID: 36725929]

F.R. Cross, P. Fridy, N. Ketaren, F. Mast, S. Li, P. Olivier, K. Pecani, B.T. Chait, J.D. Aitchison, M.P. Rout. “Expanding and Improving Nanobody Repertoires Using a Yeast Display Method: Targeting SARS-CoV-2”, J Biol Chem. 2023 Jan 28. Online ahead of print. [PMID: 36720309]

A.Y. Berman, M. Wieczorek, A. Aher, P.D.B. Olinares, B.T. Chait, T.M. Kapoor. “A nucleotide binding-independent role for γ-tubulin in microtubule capping and cell division”, J Cell Biol. 2023 Mar 6. Epub 2023 Jan 25. [PMID: 36695784]


S. Hwang, P.D.B. Olinares, J. Lee, J. Kim, B.T. Chait, R.A. King, J.Y. Kang. “Structural basis of transcriptional regulation by a nascent RNA element, HK022 putRNA”, Nat Commun. 2022 Aug 15. [Epub ahead of print] [PMID: 35970830]

J. Du, V.G. Vandavasi, K.R. Molloy, H. Yang, L.N. Massenburg, A. Singh, A.L. Kwansa, Y.G. Yingling, H. O'Neill, B.T. Chait, M. Kumar, B.T. Nixon. “Evidence for Plant-Conserved Region Mediated Trimeric CESAs in Plant Cellulose Synthase Complexes”, Biomacromolecules. 2022 Aug 10. [Online ahead of print] [PMID: 35948425]

J.C. Zinder, P.D.B. Olinares, V. Svetlov, M.W. Bush, E. Nudler, B.T. Chait, T. Walz, T. de Lange. “Shelterin is a dimeric complex with extensive structural heterogeneity”, Proc Natl Acad Sci U S A. 2022 Aug 2. 119 (31):e2201662119. [PMID: 35881804]

S. Balasubramanian, M. Andreani, J.G. Andrade, T. Saha, D. Sundaravinayagam, J. Garzón, W. Zhang, O. Popp, S.I. Hiraga, A. Rahjouei, D.B. Rosen, P. Mertins, B.T. Chait, A.D. Donaldson, M. Di Virgilio. “Protection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered region”, Elife. 2022 Apr 13;11:e75047. [PMID: 35416772]

A.A. Serganov, Y. Udi, M.E. Stein, V. Patel, P.C. Fridy, C.M. Rice, M. Saeed, E.Y. Jacobs, B.T. Chait, M.P. Rout. “Proteomic elucidation of the targets and primary functions of the picornavirus 2A protease”, J Biol Chem. 2022 Mar 30. [Epub ahead of print] [PMID: 35367208]

K.J. Mickolajczyk, P.D.B. Olinares, B.T. Chait, S. Liu, T.M. Kapoor. “The MIDAS domain of AAA mechanoenzyme Mdn1 forms catch bonds with two different substrates”, Elife. 2022 Feb 11;11:e73534. [PMID: 35147499] 


C.W. Akey, D. Singh, C. Ouch, I. Echeverria, I. Nudelman, J.M. Varberg, Z. Yu, F. Fang, Y. Shi, J. Wang, D. Salzberg, K. Song, C. Xu, J.C. Gumbart, S. Suslov, J. Unruh, S.L. Jaspersen, B.T. Chait, A. Sali, J. Fernandez-Martinez, S.J. Ludtke, E. Villa, M.P. Rout. “Comprehensive structure and functional adaptations of the yeast nuclear pore complex”, Cell. 2021 Dec 29. [Epub ahead of print] [PMID: 34982960]

F.D. Mast, P.C. Fridy, N.E. Ketaren, J. Wang, E.Y. Jacobs, J.P. Olivier, T. Sanyal, K.R. Molloy, F. Schmidt, M. Rutkowska, Y. Weisblum, L.M. Rich, E.R. Vanderwall, N. Dambrauskas, V. Vigdorovich, S. Keegan, J.B. Jiler, M.E. Stein, P.D.B. Olinares, L. Herlands, T. Hatziioannou, D.N. Sather, J.S. Debley, D. Fenyö, A. Sali, P.D. Bieniasz, J.D. Aitchison, B.T. Chait, M.P. Rout. “Highly synergistic combinations of nanobodies that target SARS-CoV-2 and are resistant to escape”, Elife. 2021 Dec 7;10:e73027. [PMID: 34874007]

Z. Hakhverdyan, K.R. Molloy, R.I. Subbotin, J. Fernandez-Martinez, B.T. Chait, M.P. Rout. “Measuring in vivo protein turnover and exchange in yeast macromolecular assemblies”, STAR Protoc. 2021 Sep 8. [PMID: 34527957]

Y. Niu, X. Tao, G. Vaisey, P.D.B. Olinares, H. Alwaseem, B. Chait, R. MacKinnon. “Analysis of the Mechanosensor Channel Functionality of TACAN”, Elife. 2021 Aug 10;10:e71188. [PMID: 34374644]

B. Bosch, M.A. DeJesus, N.C. Poulton, W. Zhang, C.A. Engelhart, A. Zaveri, S. Lavalette, N. Ruecker, C. Trujillo, J.B. Wallach, S. Li, S. Ehrt, B.T. Chait, D. Schnappinger, J.M. Rock. “Genome-wide gene expression tuning reveals diverse vulnerabilities of M. tuberculosis”, Cell. 2021 Jul 16. [Epub ahead of print] [PMID: 34297925]

B. Malone, J. Chen, Q. Wang, E. Llewellyn, Y.J. Choi, P.D.B. Olinares, X. Cao, C. Hernandez, E.T. Eng, B.T. Chait, D.E. Shaw, R. Landick, S.A. Darst, E.A. Campbell. “Structural basis for backtracking by the SARS-CoV-2 replication-transcription complex”, Proc Natl Acad Sci U S A. 2021 May 11. [PMID: 33883267]

M. Wieczorek, S.C. Ti, L. Urnavicius, K.R. Molloy, A. Aher, B.T. Chait, T.M. Kapoor. "Biochemical reconstitutions reveal principles of human γ-TuRC assembly and function.", J Cell Biol. 2021 Mar 1;220(3):e202009146. [PMID: 33496729]

J. Merkenschlager, S. Finkin, V. Ramos, J. Kraft, M. Cipolla, C.R. Nowosad, H. Hartweger, W. Zhang, P.D.B. Olinares, A. Gazumyan, T.Y. Oliveira, B.T. Chait, M.C. Nussenzweig. “Dynamic regulation of TFHselection during the germinal centre reaction.”, Nature. 2021 Feb 3. doi: 10.1038/s41586-021-03187-x. [PMID: 33536617]

J.Y. Kang, E. Llewellyn, J. Chen, P.D.B. Olinares, J. Brewer, B.T. Chait, E.A. Campbell, S.A. Darst. “Structural basis for transcription complex disruption by the Mfd translocase.”, Elife. 2021 Jan 22;10:e62117. [PMID: 33480355]

J. Xu, X. Zhao, F. Mao, V. Basrur, B. Ueberheide, B.T. Chait, C.D. Allis, S.D. Taverna, S. Gao, W. Wang, Y. Liu. “A Polycomb repressive complex is required for RNAi-mediated heterochromatin formation and dynamic distribution of nuclear bodies.”, Nucleic Acids Res. 2021 Jan 8:gkaa1262. [PMID: 33412588]

M.A. Schureck, J.E. Darling, A. Merk, J. Shao, G. Daggupati, P. Srinivasan, P.D.B. Olinares, M.P. Rout, B.T. Chait, K. Wollenberg, S. Subramaniam, S.A. Desai. “Malaria parasites use a soluble RhopH complex for erythrocyte invasion and an integral form for nutrient uptake.”, Elife. 2021 Jan 4;10:e65282. [PMID: 33393463]


Z. Hakhverdyan, K.R. Molloy, S. Keegan, T. Herricks, D.M. Lepore, M. Munson, R.I. Subbotin, D. Fenyö, J.D. Aitchison, J. Fernandez-Martinez, B.T. Chait, M.P. Rout. Dissecting the structural dynamics of the nuclear pore complex.” Mol Cell. 2020 Dec 8:S1097-2765(20)30832-7. [PMID: 33333016]

P.D.B. Olinares, J.Y. Kang, E. Llewellyn, C. Chiu, J. Chen, B. Malone, R.M. Saecker, E.A. Campbell, S.A. Darst, B.T. Chait. “Native mass spectrometry-based screening for optimal sample preparation in single-particle cryo-EM.” Structure. 2020 Nov 17:S0969-2126(20)30413-5. [PMID: 33217329]

F.D. Mast, A.T. Navare, A.M. van der Sloot, J. Coulombe-Huntington, M.P. Rout, N.S. Baliga, A. Kaushansky, B.T. Chait, A. Aderem, C.M. Rice, A. Sali, M. Tyers, J.D. Aitchison. "Crippling life support for SARS-CoV-2 and other viruses through synthetic lethality", J Cell Biol. 2020 Oct 5. 219 (10): e202006159. [PMID: 32785687]

Y. Dou, I. Barbosa, H. Jiang, C. Iasillo, K.R. Molloy, W.M. Schulze, S. Cusack, M. Schmid, H. Le Hir, J. LaCava, T.H. Jensen. “NCBP3 positively impacts mRNA biogenesis”, Nucleic Acids Res. 2020 Oct 9. [PMID: 32960271]

Y. Dou, S. Kalmykova, M. Pashkova, M. Oghbaie, H. Jiang, K.R. Molloy, B.T. Chait, M.P. Rout, D. Fenyö, T.H. Jensen, I. Altukhov, J. LaCava. "Affinity proteomic dissection of the human nuclear cap-binding complex interactome", Nucleic Acids Res. 2020 Sep 22:gkaa743. [PMID: 32960270]

J. Chen, B. Malone, E. Llewellyn, M. Grasso, P.M.M. Shelton, P.D.B. Olinares, K. Maruthi, E. Eng, H. Vatandaslar, B.T. Chait, T. Kapoor, S.A. Darst, E.A. Campbell. “Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex”, Cell. 2020 Sep 17;182(6):1560-1573.e13. [PMID: 32783916]

K.J. Mickolajczyk, P.D.B. Olinares, Y. Niu, N. Chen, S.E. Warrington, Y. Sasaki, T. Walz, B.T. Chait, T.M. Kapoor. “Long-range intramolecular allostery and regulation in the dynein-like AAA protein Mdn1”, Proc Natl Acad Sci U S A. 2020 Aug 4;117(31):18459-18469. [PMID: 32694211]

J.M. Manning, L.R. Manning, A. Dumoulin, J.C. Padovan, B.T. Chait. “Embryonic and fetal human hemoglobins: structures, oxygen binding, and physiological roles”, Subcell Biochem. 2020;94:275-296. [PMID: 32189304]

J. Kagan, R.L. Moritz, R. Mazurchuk, J.H. Lee, P.V. Kharchenko, O. Rozenblatt-Rosen, E. Ruppin, F. Edfors, F. Ginty, Y. Goltsev, J.A. Wells, J. LaCava, J.L. Riesterer, R.N. Germain, T. Shi, M.S. Chee, B.A. Budnik, J.R. Yates 3rd, B.T. Chait, J.R. Moffitt, R.D. Smith, S. Srivastava . “National Cancer Institute Think-Tank Meeting Report on proteomic cartography and biomarkers at the single-cell level: interrogation of premalignant lesions”, J Proteome Res. 2020 May 1;19(5):1900-1912. [PMID: 32163288]

S.J. Ganesan, M.J. Feyder, I.E. Chemmama, F. Fang, M.P. Rout, B.T. Chait, Y. Shi, M. Munson, A. Sali. “Integrative structure and function of the yeast exocyst complex”, Protein Sci. 2020 Jun;29(6):1486-1501. [PMID: 32239688]

J. Chen, C. Chiu, S. Gopalkrishnan, A.Y. Chen, P.D.B. Olinares, R.M. Saecker, J.T. Winkelman, M.F. Maloney, B.T. Chait, W. Ross, R.L. Gourse, E.A. Campbell, S.A. Darst. “Stepwise Promoter Melting by Bacterial RNA Polymerase”, Mol Cell. 2020 Mar 2. [Epub ahead of print] [PMID: 32160514]

V. Kieuvongngam, P.D.B. Olinares, A. Palillo, M.L. Oldham, B.T. Chait, J. Chen. “Structural basis of substrate recognition by a polypeptide processing and secretion transporter”, Elife. 2020 Jan 14. [PMID: 31934861]

P.D.B. Olinares, B.T. Chait. “Native Mass Spectrometry Analysis of Affinity-Captured Endogenous Yeast RNA Exosome Complexes”, Methods Mol Biol. 2062 (2020) 357-382. [PMID: 31768985]


D. Ardeljan, X. Wang, M. Oghbaie, M.S. Taylor, D. Husband, V. Deshpande, J.P. Steranka, M. Gorbounov, W.R. Yang, B. Sie, H.B. Larman, H. Jiang, K.R. Molloy, I. Altukhov, Z. Li, W. McKerrow, D. Fenyö, K.H. Burns, J. LaCava. “LINE-1 ORF2p expression is nearly imperceptible in human cancers”, Mob DNA. 2019 Dec 31. [PMID: 31892958]

M. Wieczorek, L. Urnavicius, S.C. Ti, K.R. Molloy, B.T. Chait, T.M. Kapoor. “Asymmetric Molecular Architecture of the Human γ-Tubulin Ring Complex”, Cell. 2019 Dec 13. [PMID: 31862189] [Epub ahead of print]

S. Presslee, G.J. Slater, F. Pujos, A.M. Forasiepi, R. Fischer, K. Molloy, M. Mackie, J.V. Olsen, A. Kramarz, M. Taglioretti, F. Scaglia, M. Lezcano, J. L. Lanata, J. Southon, R. Feranec, J. Bloch, A. Hajduk, F.M. Martin, R. Salas Gismondi, M. Reguero, C. de Muizon, A. Greenwood, B.T. Chait, K. Penkman, M. Collins, R.D.E. MacPhee. “Palaeoproteomics resolves sloth relationships”, Nat Ecol Evol. 2019 Jun 6. [Epub ahead of print] [PMID: 31171860]

R. Hayama, M. Sorci, J.J. Keating IV, L.M. Hecht, J.L. Plawsky, G. Belfort, B.T. Chait, M.P. Rout. “Interactions of nuclear transport factors and surface-conjugated FG nucleoporins: Insights and limitations”, PLoS One. 2019 Jun 6. e0217897. [PMID: 31170242]

H. Jiang, M.S. Taylor, K.R. Molloy, I. Altukhov, J. LaCava. “Identification of RNase-sensitive LINE-1 Ribonucleoprotein Interactions by Differential Affinity Immobilization”, Bio Protoc. 2019 Apr 5. [PMID: 31106238]








































2024T. Ge, D.G. Brickner, K. Zehr, D.J. VanBelzen, W. Zhang, C. Caffalette, S. Ungerleider, N. Marcou, B. Chait, M.P. Rout, J.H. Brickner. “Exportin-1 functions as an adaptor for transcription