Supplementary Video 5 (mpg 520K)
Centrosomal and nuclear motion in a stationary neuron. Purified cerebellar granule neurons were labeled with Ven-mPar6 and cultured with cerebellar glia. The neuron depicted in Supplementary Video 4 later stalls and was imaged again to observe centrosomal motion in a stationary neuron. The centrosome and nucleus oscillated non-productively when the cell was stationary, a stark contrast to what was observed when the cells was in motion. Images from this sequence were used in Fig. 4b. Total elapsed time was nearly 6 min.
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