ACF7 Mediates Microtubule Targeting to Focal Adhesions Along Actin Filaments

Through a comprehensive approach encompassing biochemistry, molecular and cell biology, we unveiled a hitherto unrecognized role of ACF7 as an essential molecular goliath that integrates coordinated microtubule and actin dynamics, promotes microtubule targeting to focal adhesions and in turn enhances focal adhesion turnover and cell migration (see Wu et al. Cell 135, 137-148 2008).

In this video, WT and KO keratinocytes were co-transfected with plasmids encoding GFP-EB1 (green, label microtubule plus-ends), DsRed-Zyxin (red, label focal adhesions) and CFP-actin (blue, label F-actin). Cytoskeletal coordination and targeting of microtubules to focal adhesions were then examined by multi-channel confocal spinning-disc videomicroscopy. Images were taken at 1 frame every 3 seconds.

ACF7 Mediates Microtubule Targeting to Focal Adhesions Along Actin FilamentsThrough a comprehensive approach encompassing biochemistry, molecular and cell biology, we unveiled a hitherto unrecog