An ACF7 minigene containing the actin and microtubule binding domains can rescue microtubule tethering to the actin cortex.

Here, we transfect ACF7 null (KO) cells with a GFP-ACF7 minigene, a fluorescent fusion protein containing the microtubule and actin binding domains of ACF7. The cells for this movie were visualized live. Note that the microtubules now tether to the actin cytoskeleton, in contrast to the KO cells in the Movie above.

An ACF7 minigene containing the actin and microtubule binding domains can rescue microtubule tethering to the actin cortex.Here, we transfect ACF7 null (KO) cells with a GFP-ACF7 minigene, a fl