Movie S2. Assembly and Dynamics of an Integrated Cytoskeleton throughout an Epidermal Sheet

Freshly isolated epidermal keratinocytes were isolated from GFP-actin expressing normal or a-catenin null mice, and plated under conditions to promote cell-cell adhesion. Note that normal keratinocytes use the actin cytoskeletal connections to adherens junctions in order to coordinate movements within a developing sheet of interconnected epithelial cells. In contrast, mice lacking a-catenin (KO) cannot connect the actin cytoskeleton to adherens junctions, and consequently, they don’t form cohesive sheets of epithelial cells. For the complete story, see Vaezi et al. (2002).
   

Movie S2. Assembly and Dynamics of an Integrated Cytoskeleton throughout an Epidermal SheetFreshly isolated epidermal keratinocytes were isolated from GFP-actin expressing normal or a-catenin null