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Publications

2017

Protein S-palmitoylation in cellular differentiation.
Zhang MM, Hang HC. Biochem Soc Trans. 2017, in press.

Mass-tag labeling for the determination of protein S-fatty acylation.
Thinon E, Percher A, Hang HC. Curr Prot Protein Sci 2017, in press.

Chemical proteomics reveals ADP-ribosylation of small GTPases during oxidative stress
Westcott NP, Fernandez JP, Molina H, Hang HC. Nat Chem Biol. 2017, online.

 

2016

Site-Specific Bioorthogonal Labeling for Fluorescence Imaging of Intracellular Proteins in Living Cells.
Peng T, Hang HC. J Am Chem Soc. 2016 Nov 2;138(43):14423-14433.

Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins.
Thinon E, Percher A, Hang HC. Chembiochem. 2016, Oct 4;17(19):1800-1803.

A secreted bacterial peptidoglycan hydrolase enhances tolerance to enteric pathogens.
Rangan KJ, Pedicord VA, Wang Y-C, Kim B, Lu Y, Shaham S, Mucida D, Hang HC. Science 2016, 353, 1434-1437.

Exploiting a host-commensal interaction to promote intestinal barrier function and enteric pathogen tolerance.
Pedicord VA, Lockhart AA, Rangan KJ, Craig JW, Loschko J, Rogoz A, Hang HC, Mucida D, Science Immunology 2016, 1, eaai7732.

Mass-tag labeling reveals site-specific and endogenous levels of protein S-fatty acylation.
Percher A, Ramakrishnan S, Thinon E, Yuan X, Yount JS, Hang HC. Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):4302-7. 

Antibacterial Flavonoids from Medicinal Plants Covalently Inactivate Type III Protein Secretion Substrates.
Tsou LK, Lara-Tejero M, RoseFigura J, Zhang ZJ, Wang YC, Yount JS, Lefebre M, Dossa PD, Kato J, Guan F, Lam W, Cheng YC, Galán JE, Hang HC. J Am Chem Soc. 2016 Feb 24;138(7):2209-18.

Proteomic analysis of fatty-acylated proteins.
Peng T, Thinon E, Hang HC. Curr Opin Chem Biol. 2016 Feb;30:77-86.

 

2015

A Single Protein S-acyl Transferase Acts through Diverse Substrates to Determine Cryptococcal Morphology, Stress Tolerance, and Pathogenic Outcome.
Santiago-Tirado FH, Peng T, Yang M, Hang HC, Doering TL. PLoS Pathog. 2015 May 13;11(5):e1004908. 

Myristoylome profiling reveals a concerted mechanism of ARF GTPase deacylation by the bacterial protease IpaJ.
Burnaevskiy N, Peng T, Reddick LE, Hang HC, Alto NM. Mol Cell. 2015 Apr 2;58(1):110-22. 

Chemical reporters for exploring protein acylation.
Thinon E, Hang HC. Biochem Soc Trans. 2015 Apr;43(2):253-61  

Bifunctional fatty acid chemical reporter for analyzing S-palmitoylated membrane protein-protein interactions in mammalian cells.
Peng T, Hang HC. J Am Chem Soc. 2015 Jan 21;137(2):556-9. 

Integrative chemical biology approaches for identification and characterization of "erasers" for fatty-acid-acylated lysine residues within proteins.
Liu Z, Yang T, Li X, Peng T, Hang HC, Li XD. Angew Chem Int Ed Engl. 2015 Jan 19;54(4):1149-52

 

2014

Chemical reporters for exploring ADP-ribosylation and AMPylation at the host-pathogen interface.
Westcott NP, Hang HC. Curr Opin Chem Biol. 2014 Dec;23:56-62.

Chemoproteomics reveals Toll-like receptor fatty acylation.
Chesarino NM, Hach JC, Chen JL, Zaro BW, Rajaram MV, Turner J, Schlesinger LS, Pratt MR, Hang HC, Yount JS. BMC Biol. 2014 Nov 5;12:91.

Copper-catalyzed azide-alkyne cycloaddition (click chemistry)-based detection of global pathogen-host AMPylation on self-assembled human protein microarrays.
Yu X, Woolery AR, Luong P, Hao YH, Grammel M, Westcott N, Park J, Wang J, Bian X, Demirkan G, Hang HC, Orth K, LaBaer J. Mol Cell Proteomics. 2014 Nov;13(11):3164-76. 

Turning the spotlight on protein-lipid interactions in cells.
Peng T, Yuan X, Hang HC. Curr Opin Chem Biol. 2014 Aug;21:144-53. 

SORTing out cellular proteomes in vivo.
Peng T, Hang HC. Nat Biotechnol. 2014 May;32(5):445-6.

 

2013

Chemical reporters for biological discovery.
Grammel M, Hang HC. Nat Chem Biol. 2013 Aug;9(8):475-84. PMID: 23868317

Quantitative Control Protein S-Palmitoylation Regulates Meiotic Entry in Fission Yeast. 
Zhang M, Wu P-YJ, Kelly FD, Nurse P, Hang HC. PLoS Biol 2013, 11(7), e10011597. PMID: 23843742  

Prenylome profiling reveals S-farnesylation is crucial for membrane targeting and antiviral activity of ZAP long-isoform.
Charron G, Li M, MacDonald M, Hang HC.  Proc. Nat. Acad. Sci. 2013, 110, 11085-90. PMID: 23776219

SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine.
Jiang H, Khan S, Wang Y, Charron G, He B, Sebastian C, Du J, Kim R, Ge E, Mostoslavsky R, Hang HC, Hao Q, Lin H. Nature. 2013 Apr 4;496(7443):110-3. PMID: 23552949  

Identification of lysine acetyltransferase substrates using bioorthogonal chemical proteomics.
Grammel M, Hang HC. Methods Mol Biol. 2013;981:201-10. PMID: 23381864 

Emerging roles for protein S-palmitoylation in immunity from chemical proteomics.
Yount JS, Zhang MM, Hang HC. Curr Opin Chem Biol. 2013 Jan 14;S1367-5931(12)00145-7. PMID: 23332315 

Incorporation of unnatural sugars for the identification of glycoproteins.
Zaro BW, Hang HC, Pratt MR. Methods Mol Biol. 2013;951:57-67. PMID: 23296524 

Small molecules aimned at type III secretion systems to inhibit bacterial virulence.
Tsou LK, Dossa PD, Hang HC. Med Chem Commun. 2013;4:68-79.

 

2012

Tiki1 Is Required for Head Formation via Wnt Cleavage-Oxidation and Inactivation.
Zhang X, Abreu JG, Yokota C, Macdonald BT, Singh S, Coburn KL, Cheong SM, Zhang MM, Ye QZ, Hang HC, Steen H, He X. Cell. 2012 Jun 22;149(7):1565-77. PMID: 22726442 

Chemical reporters for monitoring RNA synthesis and poly(a) tail dynamics.
Grammel M, Hang H, Conrad NK. Chembiochem. 2012 May 29;13(8):1112-5. PMID: 22513998

S-Palmitoylation and Ubiquitination Differentially Regulate Interferon-induced Transmembrane Protein 3 (IFITM3)-mediated Resistance to Influenza Virus.
Yount JS, Karssemeijer RA, Hang HC. J Biol Chem. 2012 Jun 1;287(23):19631-41. PMID: 22511783 

Cell-selective labeling of bacterial proteomes with an orthogonal phenylalanine amino acid reporter.
Grammel M, Dossa PD, Taylor-Salmon E, Hang HC. Chem Commun (Camb). 2012 Feb 1;48(10):1473-4. PMID: 22080199

Chemical reporters of protein methylation and acetylation
Grammel M, Hang HC. Epigenomics: From Chromatin Biology to Therapeutics; Appasani, K. Ed.; Cambridge University Press 2012

 

2011

A Chemical Reporter for Protein AMPylation.
Grammel M*, Luong P*, Orth K, Hang HC. J Am Chem Soc. 2011 Nov 2;133(43):17103-5. PMID: 21942216

  • These authors contributed equally to this manuscript.

Palmitoylation of MICA, a ligand for NKG2D, mediates its recruitment to membrane microdomains and promotes its shedding.
Agüera-González S, Gross CC, Fernández-Messina L, Ashiru O, Esteso G, Hang HC, Reyburn HT, Long EO, Valés-Gómez M. Eur J Immunol. 2011 Dec;41(12):3667-76. PMID: 21928280 

Exploring Protein Lipidation with Chemical Biology.
Hang HC, Linder ME. Chem Rev. 2011 Oct 12;111(10):6341-58. PMID: 21919527 

Subcellular targeting of Salmonella virulence proteins by host-mediated S-palmitoylation.
Hicks SW, Charron G, Hang HC, Galán JE. Cell Host Microbe. 2011 Jul 21;10(1):9-20. PMID: 21767808 

Bioorthogonal Chemical Reporters for Analyzing Protein Lipidation and Lipid Trafficking.
Hang HC, Wilson JP, Charron G. Acc Chem Res. 2011 Sep 20;44(9):699-708. PMID: 21675729 

Identification of lysine acetyltransferase p300 substrates using 4-pentynoyl-coenzyme A and bioorthogonal proteomics.
Yang Y-Y, Grammel M, Hang HC. Bioorg Med Chem Lett. 2011 Sep 1;21(17):4976-9  PMID: 21669532  

Chemical reporters for fluorescent detection and identification of O-GlcNAc-modified proteins reveal glycosylation of the ubiquitin ligase NEDD4-1.
Zaro BW, Yang YY, Hang HC, Pratt MR. Proc Natl Acad Sci U S A. 2011 May 17;108(20):8146-51. PMID: 21540332  

Visualization and Identification of Fatty Acylated Proteins Using Chemical Reporters.
Yount JS, Zhang MM, Hang HC. Curr Protoc Chem Biol. 2011 May 1;3(2):65-79. 

Bioorthogonal proteomics of 15-hexadecynyloxyacetic acid chemical reporter reveals preferential targeting of fatty acid modified proteins and biosynthetic enzymes.
Yount JS, Charron G, Hang HC. Bioorg Med Chem. 2011 Apr 3. PMID: 21524915.  

Differential Regulation of Two Palmitoylation Sites in the Cytoplasmic Tail of the beta1-Adrenergic Receptor.
Zuckerman DM, Hicks SW, Charron G, Hang HC, Machamer CE. J Biol Chem. 2011 May 27;286(21):19014-23. PMID: 21464135 

Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.
Wilson JP, Raghavan AS, Yang YY, Charron G, Hang HC. Mol Cell Proteomics. 2011 Mar;10(3):M110.001198. PMID: 21076176.

Chemical approaches for the detection and synthesis of acetylated proteins.
Yang YY, Hang HC. Chembiochem. 2011 Jan 24;12(2):314-22. PMID: 21243719.  

Alkynyl-farnesol reporters for detection of protein S-prenylation in cells.
Charron G, Tsou LK, Maguire W, Yount JS, Hang HC. Mol Biosyst. 2011 Jan 1;7(1):67-73. PMID: 21107478.

 

2010

Comparative analysis of cleavable azobenzene-based affinity tags for bioorthogonal chemical proteomics.
Yang YY, Grammel M, Raghavan AS, Charron G, Hang HC. Chem Biol. 2010 Nov 24;17(11):1212-22. PMID: 21095571. 

Lipidation by the host prenyltransferase machinery facilitates membrane localization of Legionella pneumophila effector proteins.
Ivanov SS, Charron G, Hang HC, Roy CR. J Biol Chem. 2010 Nov 5;285(45):34686-98. PMID: 20813839.  

Orthogonal Alkynyl Amino Acid Reporter for Selective Labeling of Bacterial Proteomes during Infection.
Grammel M, Zhang MM, Hang HC. Angew Chem Int Ed Engl. 2010 Jul 14. PMID: 20632346. 

Palmitoylome profiling reveals S-palmitoylation-dependent antiviral activity of IFITM3.
Yount JS, Moltedo B, Yang YY, Charron G, Moran TM, López CB, Hang HC. Nat Chem Biol. 2010 Aug;6(8):610-4. PMID: 20601941.  

Visible fluorescence detection of type III protein secretion from bacterial pathogens.
Yount JS, Tsou LK, Dossa PD, Kullas AL, van der Velden AW, Hang HC. J Am Chem Soc. 2010 Jun 23;132(24):8244-5. PMID: 20504019.

Tandem fluorescence imaging of dynamic S-acylation and protein turnover.
Zhang MM*, Tsou LK*, Charron G, Raghavan AS, Hang HC. Proc Natl Acad Sci U S A. 2010 May 11;107(19):8627-32. PMID: 20421494.

  • These authors contributed equally to this manuscript  

Rapid visualization and large-scale profiling of bacterial lipoproteins with chemical reporters.
Rangan KJ, Yang YY, Charron G, Hang HC. J Am Chem Soc. 2010 Aug 11;132(31):10628-9. PMID: 20230003.

Bioorthogonal chemical reporters for monitoring protein acetylation.
Yang YY, Ascano JM, Hang HC. J Am Chem Soc. 2010 Mar 24;132(11):3640-1. PMID: 20192265.  

Molecular Probes of Protein Glycosylation.
Hang, HC. Comprehensive Natural Products II Chemistry and Biology; Mander, L., Lui, H.-W, Eds.; Elsevier: Oxford, 2010.

 

2009

Clickable fluorescent dyes for multimodal bioorthogonal imaging.
Tsou LK, Zhang MM, Hang HC. Org Biomol Chem. 2009 Dec 21;7(24):5055-8. PMID: 20024096. 

Chemical tools for understanding protein lipidation in eukaryotes.
Charron G, Wilson J, Hang HC. Curr Opin Chem Biol. 2009 Oct;13(4):382-91. PMID: 19699139. 

Seeing small molecules in action with bioorthogonal chemistry.
Raghavan AS, Hang HC. Drug Discov Today. 2009 Feb;14(3-4):178-84. PMID: 18973827.

Robust fluorescent detection of protein fatty-acylation with chemical reporters.
Charron G, Zhang MM, Yount JS, Wilson J, Raghavan AS, Shamir E, Hang HC. J Am Chem Soc. 2009 Apr 8;131(13):4967-75. PMID: 19281244.

 

2008

Chemical probes for profiling fatty acid-associated proteins in living cells.
Raghavan A, Charron G, Flexner J, Hang HC.
Bioorg Med Chem Lett. 2008 Nov 15;18(22):5982-6. PMID: 18929483 

Lipid-independent secretion of a Drosophila Wnt protein.
Ching W, Hang HC, Nusse R. J Biol Chem. 2008 Jun 20;283(25):17092-8. PMID: 18430724. 

 

Hang Postdoctoral & Graduate Publications

Lysosomal cysteine and aspartic proteases are heterogeneously expressed and act redundantly to initiate human invariant chain degradation.
Costantino CM, Hang HC, Kent SC, Hafler DA, Ploegh HL. J Immunol. 2008 Mar 1;180(5):2876-85. PMID: 18292509.  

Chemical probes for the rapid detection of Fatty-acylated proteins in Mammalian cells.
Hang HC, Geutjes EJ, Grotenbreg G, Pollington AM, Bijlmakers MJ, Ploegh HL. J Am Chem Soc. 2007 Mar 14;129(10):2744-5. PMID: 17305342.

Mechanism-based probe for the analysis of cathepsin cysteine proteases in living cells.
Hang HC, Loureiro J, Spooner E, van der Velden AW, Kim YM, Pollington AM, Maehr R, Starnbach MN, Ploegh HL. ACS Chem Biol. 2006 Dec 20;1(11):713-23. PMID: 17184136. 

The chemistry and biology of mucin-type O-linked glycosylation.
Hang HC, Bertozzi CR. Bioorg Med Chem. 2005 Sep 1;13(17):5021-34. Review. PMID: 16005634.

Asparagine endopeptidase is not essential for class II MHC antigen presentation but is required for processing of cathepsin L in mice.
Maehr R, Hang HC, Mintern JD, Kim YM, Cuvillier A, Nishimura M, Yamada K, Shirahama-Noda K, Hara-Nishimura I, Ploegh HL. J Immunol. 2005 Jun 1;174(11):7066-74. PMID: 15905550.  

Catching proteases in action with microarrays.
Hang HC, Ploegh H. Chem Biol. 2004 Oct;11(10):1328-30. Review. PMID: 15489158. 

Deconvoluting the functions of polypeptide N-alpha-acetylgalactosaminyltransferase family members by glycopeptide substrate profiling.
Pratt MR, Hang HC, Ten Hagen KG, Rarick J, Gerken TA, Tabak LA, Bertozzi CR. Chem Biol. 2004 Jul;11(7):1009-16. PMID: 15271359 

Small molecule inhibitors of mucin-type O-linked glycosylation from a uridine-based library.
Hang HC, Yu C, Ten Hagen KG, Tian E, Winans KA, Tabak LA, Bertozzi CR. Chem Biol. 2004 Mar;11(3):337-45. PMID: 15123263.

Probing glycosyltransferase activities with the Staudinger ligation.
Hang HC, Yu C, Pratt MR, Bertozzi CR. J Am Chem Soc. 2004 Jan 14;126(1):6-7. PMID: 14709032. 

A metabolic labeling approach toward proteomic analysis of mucin-type O-linked glycosylation.
Hang HC, Yu C, Kato DL, Bertozzi CR. Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14846-51. PMID: 14657396. 

cDNA cloning and expression of UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase T1 from Toxoplasma gondii.
Wojczyk BS, Stwora-Wojczyk MM, Hagen FK, Striepen B, Hang HC, Bertozzi CR, Roos DS, Spitalnik SL. Mol Biochem Parasitol. 2003 Oct;131(2):93-107. PMID: 14511808.

Constructing azide-labeled cell surfaces using polysaccharide biosynthetic pathways.
Luchansky SJ, Hang HC, Saxon E, Grunwell JR, Yu C, Dube DH, Bertozzi CR. Methods Enzymol. 2003;362:249-72. PMID: 12968369  

A chemical approach for identifying O-GlcNAc-modified proteins in cells.
Vocadlo DJ, Hang HC, Kim EJ, Hanover JA, Bertozzi CR. Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9116-21. PMID: 12874386.  

Investigating cellular metabolism of synthetic azidosugars with the Staudinger ligation.
Saxon E, Luchansky SJ, Hang HC, Yu C, Lee SC, Bertozzi CR. J Am Chem Soc. 2002 Dec 18;124(50):14893-902.  PMID: 12475330.

Substrate specificity of the sialic acid biosynthetic pathway.
Jacobs CL, Goon S, Yarema KJ, Hinderlich S, Hang HC, Chai DH, Bertozzi CR. Biochemistry. 2001 Oct 30;40(43):12864-74. PMID: 11669623.  

Chemoselective approaches to glycoprotein assembly.
Hang HC, Bertozzi CR. Acc Chem Res. 2001 Sep;34(9):727-36. Review. PMID: 11560472. 

Ketone isosteres of 2-N-acetamidosugars as substrates for metabolic cell surface engineering.
Hang HC, Bertozzi CR. J Am Chem Soc. 2001 Feb 14;123(6):1242-3. PMID: 11456684.

2017Protein S-palmitoylation in cellular differentiation.Zhang MM, Hang HC. Biochem Soc Trans. 2017, in press.Mass-tag labeling for the determination of protein S-fatty acylation. Thinon E, Perche